- NBP1-32822 - Novus Biologicals NFE2L2 antibody

Submitted references The effects of epidermal growth factor on early burn-wound progression in rats.

Kaya O, Orhan E, Sapmaz-Metin M, Topçu-Tarladaçalışır Y, Gündüz Ö, Aydın B
Dermatologic therapy 2020 Jan;33(1):e13196

A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2.

Leung MH, Tsoi H, Gong C, Man EP, Zona S, Yao S, Lam EW, Khoo US
Cancers 2020 Feb 26;12(3)

Multitarget Profiling of a Strigolactone Analogue for Early Events of Alzheimer's Disease: In Vitro Therapeutic Activities against Neuroinflammation.

Kurt B, Ozleyen A, Antika G, Yilmaz YB, Tumer TB
ACS chemical neuroscience 2020 Feb 19;11(4):501-507

Possible mechanisms underlying transcriptional induction of metallothionein isoforms by tris(pentafluorophenyl)stibane, tris(pentafluorophenyl)arsane, and tris(pentafluorophenyl)phosphane in cultured bovine aortic endothelial cells.

Fujie T, Takenaka F, Yoshida E, Yasuike S, Fujiwara Y, Shinkai Y, Kumagai Y, Yamamoto C, Kaji T
The Journal of toxicological sciences 2019;44(5):327-333

Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids.

Serafini MM, Catanzaro M, Fagiani F, Simoni E, Caporaso R, Dacrema M, Romanoni I, Govoni S, Racchi M, Daglia M, Rosini M, Lanni C
Frontiers in pharmacology 2019;10:1597

Hydralazine targets cAMP-dependent protein kinase leading to sirtuin1/5 activation and lifespan extension in C. elegans.

Dehghan E, Goodarzi M, Saremi B, Lin R, Mirzaei H
Nature communications 2019 Oct 28;10(1):4905

A Protective Effect of PPARα in Endothelial Progenitor Cells Through Regulating Metabolism.

Shao Y, Chen J, Dong LJ, He X, Cheng R, Zhou K, Liu J, Qiu F, Li XR, Ma JX
Diabetes 2019 Nov;68(11):2131-2142

In vitro and in silico studies of nitrobenzamide derivatives as potential anti-neuroinflammatory agents.

Kulabas SS, Onder FC, Yılmaz YB, Ozleyen A, Durdagi S, Sahin K, Ay M, Tumer TB
Journal of biomolecular structure & dynamics 2019 Nov 5;:1-14

Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathway.

Singh R, Chandrashekharappa S, Bodduluri SR, Baby BV, Hegde B, Kotla NG, Hiwale AA, Saiyed T, Patel P, Vijay-Kumar M, Langille MGI, Douglas GM, Cheng X, Rouchka EC, Waigel SJ, Dryden GW, Alatassi H, Zhang HG, Haribabu B, Vemula PK, Jala VR
Nature communications 2019 Jan 9;10(1):89

Mitochondrial division inhibitor 1 reduces dynamin-related protein 1 and mitochondrial fission activity.

Manczak M, Kandimalla R, Yin X, Reddy PH
Human molecular genetics 2019 Jan 15;28(2):177-199

Cryptotanshinone from the Salvia miltiorrhiza Bunge Attenuates Ethanol-Induced Liver Injury by Activation of AMPK/SIRT1 and Nrf2 Signaling Pathways.

Nagappan A, Kim JH, Jung DY, Jung MH
International journal of molecular sciences 2019 Dec 30;21(1)

Anti-Diabetic Countermeasures Against Tobacco Smoke-Dependent Cerebrovascular Toxicity: Use and Effect of Rosiglitazone.

Sivandzade F, Cucullo L
International journal of molecular sciences 2019 Aug 29;20(17)

Lithocholic Acid, a Metabolite of the Microbiome, Increases Oxidative Stress in Breast Cancer.

Kovács P, Csonka T, Kovács T, Sári Z, Ujlaki G, Sipos A, Karányi Z, Szeőcs D, Hegedűs C, Uray K, Jankó L, Kiss M, Kiss B, Laoui D, Virág L, Méhes G, Bai P, Mikó E
Cancers 2019 Aug 27;11(9)

Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway.

Rungratanawanich W, Abate G, Serafini MM, Guarienti M, Catanzaro M, Marziano M, Memo M, Lanni C, Uberti D
Oxidative medicine and cellular longevity 2018;2018:2987249

GR24, a synthetic analog of Strigolactones, alleviates inflammation and promotes Nrf2 cytoprotective response: In vitro and in silico evidences.

Tumer TB, Yılmaz B, Ozleyen A, Kurt B, Tok TT, Taskin KM, Kulabas SS
Computational biology and chemistry 2018 Oct;76:179-190

Senescence marker protein 30 protects intestinal epithelial cells against inflammation-induced cell death by enhancing Nrf2 activity.

Choo J, Heo G, Kim SJ, Lee Y, Ishigami A, Maruyama N, Chung HY, Im E
Biochimica et biophysica acta. Molecular basis of disease 2018 Dec;1864(12):3668-3678

Maternal exercise upregulates mitochondrial gene expression and increases enzyme activity of fetal mouse hearts.

Chung E, Joiner HE, Skelton T, Looten KD, Manczak M, Reddy PH
Physiological reports 2017 Mar;5(5)

Rapamycin regulates macrophage activation by inhibiting NLRP3 inflammasome-p38 MAPK-NFκB pathways in autophagy- and p62-dependent manners.

Ko JH, Yoon SO, Lee HJ, Oh JY
Oncotarget 2017 Jun 20;8(25):40817-40831

Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway.

Dehghan E, Zhang Y, Saremi B, Yadavali S, Hakimi A, Dehghani M, Goodarzi M, Tu X, Robertson S, Lin R, Chudhuri A, Mirzaei H
Nature communications 2017 Dec 20;8(1):2223

Discovery, Synthesis, and Functional Characterization of a Novel Neuroprotective Natural Product from the Fruit of Alpinia oxyphylla for use in Parkinson's Disease Through LC/MS-Based Multivariate Data Analysis-Guided Fractionation.

Li G, Zhang Z, Quan Q, Jiang R, Szeto SS, Yuan S, Wong WT, Lam HH, Lee SM, Chu IK
Journal of proteome research 2016 Aug 5;15(8):2595-606

The proto-oncometabolite fumarate binds glutathione to amplify ROS-dependent signaling.

Sullivan LB, Martinez-Garcia E, Nguyen H, Mullen AR, Dufour E, Sudarshan S, Licht JD, Deberardinis RJ, Chandel NS
Molecular cell 2013 Jul 25;51(2):236-48

No comments: Submit comment

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody. The observed M.W. is based on the publication: PMID: 22703241

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) RAW264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by western blot analysis. *p < 0.05, two-tailed Student's t test, n = 3, mean +/- SD. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-017-02394-3), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Rat-2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nrf2 Antibody [NBP1-32822] - Hydralazine enhances NRF2 signaling in SH-SY5Y cells d NRF2 translocates to the nucleus with hydralazine treatment. Treated cells were subjected to cell fractionation and western blot analysis. *p < 0.05 and **p < 0.01, two-tailed Student's t test, n = 3, mean +/- SD. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-017-02394-3), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody [N2C2], Internal.. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Paraffin-embedded Cal27 xenograft, using Nrf2 antibody at 1:500 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Human breast carcinoma. NRF2 stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NRF2 stained by NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Chromatin Immunoprecipitation: Nrf2 Antibody [NBP1-32822] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

NBP1-32822

Antibody data