AF3925

antibody from R&D Systems

Targeting: NFE2L2 NRF2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3925

Provider

R&D Systems

Product name

Human/Mouse Nrf2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q16236


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 µM MG 132 (Catalog # 1748) for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.