sc-631

antibody from Santa Cruz Biotechnology

Targeting: ELF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-631

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-631, RRID:AB_631428

Product name

Anti-ELF1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Binding sites for ETS family of transcription factors dominate the promoter regions of differentially expressed genes in abdominal aortic aneurysms.

Nischan J, Gatalica Z, Curtis M, Lenk GM, Tromp G, Kuivaniemi H
Circulation. Cardiovascular genetics 2009 Dec;2(6):565-72

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Genome-wide analyses reveal properties of redundant and specific promoter occupancy within the ETS gene family.

Hollenhorst PC, Shah AA, Hopkins C, Graves BJ
Genes & development 2007 Aug 1;21(15):1882-94

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Journal of Cerebral Blood Flow and Metabolism Volume 6, 1986

Journal of Cerebral Blood Flow & Metabolism 1986 Dec;6(6):775-776

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 67

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.