PAB28481

antibody from Abnova Corporation

Targeting: ELF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28481

Provider

Abnova Corporation

Product name

ELF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ELF1

Antigen sequence

PSSSIESSDPSLSSSATSNRNQTSRSRVSSSPGVK
GGATTVLKPGNSKAAKPKDPVEVAQPSEVLRTVQP
TQSPYPTQLFRTVHVVQPVQAVPEGEAARTSTMQD
ETLNSSVQSIRTIQAPTQVPVVVSPRNQQL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ELF1 polyclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with ELF1 polyclonal antibody (Cat # PAB28481) at 1-4 ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with ELF1 polyclonal antibody (Cat # PAB28481) shows distinct nuclear positivity in germinal center cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)