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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- MAB8167 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat NKX2.2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NKX2.2 in ELISA and Western Blot.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O95096- Isotype
- IgG
- Antibody clone number
- 883411
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Integrator of Stress Responses Calmodulin Binding Transcription Activator 1 (Camta1) Regulates miR-212/miR-132 Expression and Insulin Secretion.
Mollet IG, Malm HA, Wendt A, Orho-Melander M, Eliasson L
The Journal of biological chemistry 2016 Aug 26;291(35):18440-52
The Journal of biological chemistry 2016 Aug 26;291(35):18440-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse NKX2.2 by Western Blot. Western blot shows lysates of A-673 human Ewing's sarcoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NKX2.2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NKX2.2 in A673 Human Cell Line. NKX2.2 was detected in immersion fixed A673 human Ewing sarcoma cell line using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- NKX2.2 in Rat Cortical Stem Cells. NKX2.2 was detected in immersion fixed rat cortical stem cells differentiated for 7 days using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
