PAB20216

antibody from Abnova Corporation

Targeting: NKX2-2 NKX2.2, NKX2B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20216

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20216, RRID:AB_10964613

Product name

NKX2-2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NKX2-2.

Antigen sequence

SLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEEN
EGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDN
PYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEP
SADESPDNDKETP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with NKX2-2 polyclonal antibody (Cat # PAB20216).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with NKX2-2 polyclonal antibody (Cat # PAB20216) shows strong nuclear positivity in islet cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)