Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-72761 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nkx2.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NKX2-2 in rat kidney tissue lysate with Nkx2.2 Polyclonal Antibody (Product # PA5-72761) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Nkx2.2 Polyclonal Antibody (Product # PA5-72761) and due to glycosylation a 45kDa band corresponding to NKX2.2 was observed across cell lines tested along with an uncharacterized band at ~ 50 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of Neural Stem Cell (Lane 1), U-2 OS (Lane 2), U-87 MG (Lane 3) and A549 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036), 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NKX2-2 in human kidney tissue with Nkx2.2 Polyclonal Antibody (Product # PA5-72761) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of NKX2-2 in human kidney tissue with Nkx2.2 Polyclonal Antibody (Product # PA5-72761) at 2.5 µg/mL.