PA5-78079

antibody from Invitrogen Antibodies

Targeting: NKX2-2 NKX2.2, NKX2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78079

Provider

Invitrogen Antibodies

Product name

Nkx2.2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Nkx2.2-transfected 293T

Concentration

1.13 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nkx2.2 in non-transfected (-) and transfected (+) 293T cells using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with Nkx2.2 polyclonal antibody (Product # PA5-78079) using a dilution of 1:5000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Nkx2.2 Polyclonal Antibody (Product # PA5-78079). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.2 Polyclonal Antibody (Product # PA5-78079) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Nkx2.2 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Nkx2.2 Polyclonal Antibody (Product # PA5-78079) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of Nkx2.2 was performed in frozen-sectioned rat E13.5 brain tissue using Nkx2.2 Polyclonal Antibody (Product # PA5-78079) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.