MA5-32713

antibody from Invitrogen Antibodies

Targeting: CLOCK bHLHe8, KAT13D, KIAA0334

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32713

Provider

Invitrogen Antibodies

Product name

CLOCK Recombinant Rabbit Monoclonal Antibody (JA12-33)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

JA12-33

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CLOCK Recombinant Rabbit Monoclonal Antibody (JA12-33) (Product # MA5-32713) and a 95 kDa band corresponding to CLOCK was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of U-2 OS (Lane 1), HeLa (Lane 2), A-431 (Lane 3), MCF7 (Lane 4), MCF 10A (Lane 5) and tissue extracts (30 µg lysate) of Mouse Testis (Lane 6), Rat Testis (Lane 7), Mouse Liver (Lane 8), Mouse Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CLOCK in MCF-7 (1) and PC-12 (2) cell using a CLOCK Monoclonal antibody (Product # MA5-32713) at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CLOCK in Hela cells using a CLOCK Monoclonal antibody (Product # MA5-32713) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CLOCK in SHG-44 cells using a CLOCK Monoclonal antibody (Product # MA5-32713) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CLOCK of paraffin-embedded Human fetal skeletal muscle tissue using a CLOCK Monoclonal antibody (Product #MA5-32713). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CLOCK of paraffin-embedded Mouse colon tissue using a CLOCK Monoclonal antibody (Product #MA5-32713). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CLOCK of paraffin-embedded Human colon cancer tissue using a CLOCK Monoclonal antibody (Product #MA5-32713). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CLOCK of paraffin-embedded Human kidney tissue using a CLOCK Monoclonal antibody (Product #MA5-32713). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of CLOCK in Hela cells using a CLOCK Monoclonal Antibody (Product # MA5-32713) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).