HPA023835

antibody from Atlas Antibodies

Targeting: MUC12 MUC11

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA023835

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023835, RRID:AB_1854199

Product name

Anti-MUC12

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EELFENLAEIVKAKIMNETRTTLLDPDSCRKAILC
YSEEDTFVDSSVTPGFDFQEQCTQKAAEGYTQFYY
VDVLDGKLACVNKCTKGTKSQMNCNLGTCQLQRSG
PRCLCPNTNTHWYWG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human rectum and liver tissues using HPA023835 antibody. Corresponding MUC12 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows no positivity in cells in tubules as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows moderate positivity in glandular cells of apical membrane.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows moderate positivity in apical membrane in glandular cells.

Sample type

HUMAN