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LearnH00009063-M01
antibody from Abnova Corporation
Targeting: PIAS2
ARIP3, miz, PIASX-ALPHA, PIASX-BETA, ZMIZ4
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00009063-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009063-M01, RRID:AB_489938
- Product name
- PIAS2 monoclonal antibody (M01), clone 1F7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PIAS2.
- Antigen sequence
CPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQ
EDGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPC
SVTVASEASKKKVDVIDLT- Isotype
- IgG
- Antibody clone number
- 1F7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PIAS2 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PIAS2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between TP53 and PIAS2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PIAS2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
