PAB28482

antibody from Abnova Corporation

Targeting: MZF1 MZF-1, MZF1B, Zfp98, ZNF42, ZSCAN6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28482

Provider

Abnova Corporation

Product name

MZF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MZF1

Antigen sequence

TLTQHLRVHTGEKPFACPECGQRFSQRLKLTRHQR
THTGEKPYHCGECGLGFTQVSRLTEHQRIHTGERP
FACPECGQSFRQHANLTQHRRIHTGERPYACPECG
KAFRQRPTLTQHLRTHRREKPFACQDCGRRFHQST
K


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with MZF1 polyclonal antibody at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with MZF1 polyclonal antibody (Cat # PAB28482) at 1-4 ug/ml shows positivity in nuclei.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with MZF1 polyclonal antibody (Cat # PAB28482) shows nuclear and cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)