H00006662-M02

antibody from Abnova Corporation

Targeting: SOX9 CMD1, CMPD1, SRA1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006662-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006662-M02, RRID:AB_875842

Product name

SOX9 monoclonal antibody (M02), clone 3C10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SOX9.

Antigen sequence

EQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPP
ITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYM
NPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYT
QLTRP


Isotype

IgG

Antibody clone number

3C10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

In vitro osteogenic potential of human mesenchymal stem cells is predicted by Runx2/Sox9 ratio.

Loebel C, Czekanska EM, Bruderer M, Salzmann G, Alini M, Stoddart MJ
Tissue engineering. Part A 2015 Jan;21(1-2):115-23

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Expression of cancer stem cell markers is more frequent in anaplastic thyroid carcinoma compared to papillary thyroid carcinoma and is related to adverse clinical outcome.

Yun JY, Kim YA, Choe JY, Min H, Lee KS, Jung Y, Oh S, Kim JE
Journal of clinical pathology 2014 Feb;67(2):125-33

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Supra- and infratentorial pediatric ependymomas differ significantly in NeuN, p75 and GFAP expression.

Hagel C, Treszl A, Fehlert J, Harder J, von Haxthausen F, Kern M, von Bueren AO, Kordes U
Journal of neuro-oncology 2013 Apr;112(2):191-7

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A molecular profile of focal segmental glomerulosclerosis from formalin-fixed, paraffin-embedded tissue.

Hodgin JB, Borczuk AC, Nasr SH, Markowitz GS, Nair V, Martini S, Eichinger F, Vining C, Berthier CC, Kretzler M, D'Agati VD
The American journal of pathology 2010 Oct;177(4):1674-86

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SOX9 monoclonal antibody (M02), clone 3C10 Western Blot analysis of SOX9 expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody (M02), clone 3C10.Lane 1: SOX9 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SOX9 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SOX9 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol