Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- H00006662-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006662-M02, RRID:AB_877610
- Product name
- Mouse Monoclonal SOX9 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SOX9 (3C10)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The Influence of TGF-β3, EGF, and BGN on SOX9 and RUNX2 Expression in Human Chondrogenic Progenitor Cells.
GLI1 promotes cancer stemness through intracellular signaling pathway PI3K/Akt/NFκB in colorectal adenocarcinoma.
Beyond the brain: disrupted in schizophrenia 1 regulates pancreatic β-cell function via glycogen synthase kinase-3β.
Loss of SOX9 Expression Is Associated with PSA Recurrence in ERG-Positive and PTEN Deleted Prostate Cancers.
In vitro osteogenic potential of human mesenchymal stem cells is predicted by Runx2/Sox9 ratio.
Expression of cancer stem cell markers is more frequent in anaplastic thyroid carcinoma compared to papillary thyroid carcinoma and is related to adverse clinical outcome.
Supra- and infratentorial pediatric ependymomas differ significantly in NeuN, p75 and GFAP expression.
A molecular profile of focal segmental glomerulosclerosis from formalin-fixed, paraffin-embedded tissue.
Janssen JN, Batschkus S, Schimmel S, Bode C, Schminke B, Miosge N
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2019 Feb;67(2):117-127
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2019 Feb;67(2):117-127
GLI1 promotes cancer stemness through intracellular signaling pathway PI3K/Akt/NFκB in colorectal adenocarcinoma.
Yang Z, Zhang C, Qi W, Cui Y, Xuan Y
Experimental cell research 2018 Dec 15;373(1-2):145-154
Experimental cell research 2018 Dec 15;373(1-2):145-154
Beyond the brain: disrupted in schizophrenia 1 regulates pancreatic β-cell function via glycogen synthase kinase-3β.
Jurczyk A, Nowosielska A, Przewozniak N, Aryee KE, DiIorio P, Blodgett D, Yang C, Campbell-Thompson M, Atkinson M, Shultz L, Rittenhouse A, Harlan D, Greiner D, Bortell R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Feb;30(2):983-93
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Feb;30(2):983-93
Loss of SOX9 Expression Is Associated with PSA Recurrence in ERG-Positive and PTEN Deleted Prostate Cancers.
Burdelski C, Bujupi E, Tsourlakis MC, Hube-Magg C, Kluth M, Melling N, Lebok P, Minner S, Koop C, Graefen M, Heinzer H, Wittmer C, Sauter G, Wilczak W, Simon R, Schlomm T, Steurer S, Krech T
PloS one 2015;10(6):e0128525
PloS one 2015;10(6):e0128525
In vitro osteogenic potential of human mesenchymal stem cells is predicted by Runx2/Sox9 ratio.
Loebel C, Czekanska EM, Bruderer M, Salzmann G, Alini M, Stoddart MJ
Tissue engineering. Part A 2015 Jan;21(1-2):115-23
Tissue engineering. Part A 2015 Jan;21(1-2):115-23
Expression of cancer stem cell markers is more frequent in anaplastic thyroid carcinoma compared to papillary thyroid carcinoma and is related to adverse clinical outcome.
Yun JY, Kim YA, Choe JY, Min H, Lee KS, Jung Y, Oh S, Kim JE
Journal of clinical pathology 2014 Feb;67(2):125-33
Journal of clinical pathology 2014 Feb;67(2):125-33
Supra- and infratentorial pediatric ependymomas differ significantly in NeuN, p75 and GFAP expression.
Hagel C, Treszl A, Fehlert J, Harder J, von Haxthausen F, Kern M, von Bueren AO, Kordes U
Journal of neuro-oncology 2013 Apr;112(2):191-7
Journal of neuro-oncology 2013 Apr;112(2):191-7
A molecular profile of focal segmental glomerulosclerosis from formalin-fixed, paraffin-embedded tissue.
Hodgin JB, Borczuk AC, Nasr SH, Markowitz GS, Nair V, Martini S, Eichinger F, Vining C, Berthier CC, Kretzler M, D'Agati VD
The American journal of pathology 2010 Oct;177(4):1674-86
The American journal of pathology 2010 Oct;177(4):1674-86
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody (M02), clone 3C10.Lane 1: SOX9 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - SOX9 monoclonal antibody (M02), clone 3C10 Analysis of SOX9 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SOX9 Antibody (3C10) [H00006662-M02] - Detection limit for recombinant GST tagged SOX9 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on HepG2 cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SOX9 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - analysis of SOX9 in formalin-fixed, paraffin-embedded human fetal developing pancreas tissue using anti-SOX9 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.7 ug/ml.