14-9765-80

antibody from Invitrogen Antibodies

Targeting: SOX9 CMD1, CMPD1, SRA1

Provider product page for 14-9765-80

Western blot

Immunocytochemistry

Immunohistochemistry

Chromatin Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [7]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [2]
Chromatin Immunoprecipitation [1]

Submit

Product number: 14-9765-80 - Provider product page
Provider: Invitrogen Antibodies
Product name: SOX9 Monoclonal Antibody (GMPR9), eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: This GMPR9 monoclonal antibody reacts with human and mouse Sox9, a member of the Sry HMG-box (Sox) gene family of transcription factors. Like Sry, Sox9 contains a high mobility group (HMG-box) DNA-binding domain. The Sox9 DNA-binding domain recognizes the sequence AGAACAATGG, and when bound to co-factors, functions to bend the DNA. This structural change is thought to be important for chromatin remodeling and the initiation of more broad transcriptional regulation of multiple genes. Sox9 is expressed in multiple tissue types during development and its activation of downstream targets is determined by interaction with tissue-specific co-factors. During development, Sox9 expression is important for cartilage formation (mesenchymal to chondrocyte), sex determination (testes formation), neural crest formation and retinal development. Sox9 plays a role in the differentiation of multiple cell types including glial, hair, cardiac, melanocytes, pancreatic, prostate, and intestinal epithelium. Sox9 regulates expression of the following family member genes sox5, sox6, and sox10, in addition to collagen type 2 alpha, collagen 9A1, CEACAM1, and snail2. Sox9 dysregulation is involved in craniofacial defects, cleft palate, sex reversal, and some types of cancer (melanoma, lung adenocarcinoma, and breast cancer). Sox9 has also been shown to be expressed in adult stem cells in the liver, pancreas and intestine. Applications Reported: This GMPR9 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This GMPR9 antibody has been tested by immunohistochemistry of formalin fixed paraffin embedded human tissue using high or low pH antigen retrieval and can be used at less than or equal to 1 µg/mL. This GMPR9 antibody has been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: GMPR9
Vial size: 25 µg
Concentration: 0.5 mg/mL
Storage: 4° C

SOX9 protein structure - 14-9765-80 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SOX9 was achieved by transfecting HCT 116 with SOX9 specific siRNAs (Silencer® select Product # S13306, S13307). Western blot analysis (Fig. a) was performed using nuclear enriched extracts from the SOX9 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SOX9 Monoclonal Antibody (GMPR9), eBioscience™ (Product # 14-9765-82, 0.5 ug/ml) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SOX9.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SOX9 Monoclonal Antibody (GMPR9), eBioscience™(Product # 14-9765-82) and a 60 kDa band corresponding to SOX9 was observed across HCT 116, U-2 OS, Hep G2 and NIH/3T3. Nuclear enriched extracts (30 µg lysate) of HCT 116 (Lane 1), U-2 OS (Lane 2), Hep G2 (Lane 3), Daudi (Lane 4), THP-1 (Lane 5) and NIH/3T3 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).