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- References [13]
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- Immunohistochemistry [6]
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- Product number
- NBP1-85551 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85551, RRID:AB_11002706
- Product name
- Rabbit Polyclonal SOX9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SOX9 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Lipid availability determines fate of skeletal progenitor cells via SOX9.
Nestin-GFP transgene labels skeletal progenitors in the periosteum.
The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure.
FGF signaling patterns cell fate at the interface between tendon and bone.
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Bioinspired seeding of biomaterials using three dimensional microtissues induces chondrogenic stem cell differentiation and cartilage formation under growth factor free conditions.
Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury.
Porcine SOX9 Gene Expression Is Influenced by an 18 bp Indel in the 5'-Untranslated Region.
Prognostic Significance of β-Catenin, E-Cadherin, and SOX9 in Colorectal Cancer: Results from a Large Population-Representative Series.
Sox9 mediates Notch1-induced mesenchymal features in lung adenocarcinoma.
Cell differentiation versus cell death: extracellular glucose is a key determinant of cell fate following oxidative stress exposure.
Evaluation of protein biomarkers of prostate cancer aggressiveness.
Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver.
van Gastel N, Stegen S, Eelen G, Schoors S, Carlier A, Daniëls VW, Baryawno N, Przybylski D, Depypere M, Stiers PJ, Lambrechts D, Van Looveren R, Torrekens S, Sharda A, Agostinis P, Lambrechts D, Maes F, Swinnen JV, Geris L, Van Oosterwyck H, Thienpont B, Carmeliet P, Scadden DT, Carmeliet G
Nature 2020 Mar;579(7797):111-117
Nature 2020 Mar;579(7797):111-117
Nestin-GFP transgene labels skeletal progenitors in the periosteum.
Tournaire G, Stegen S, Giacomini G, Stockmans I, Moermans K, Carmeliet G, van Gastel N
Bone 2020 Apr;133:115259
Bone 2020 Apr;133:115259
The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure.
Cheng B, Liu Y, Zhao Y, Li Q, Liu Y, Wang J, Chen Y, Zhang M
Scientific reports 2019 Sep 2;9(1):12642
Scientific reports 2019 Sep 2;9(1):12642
FGF signaling patterns cell fate at the interface between tendon and bone.
Roberts RR, Bobzin L, Teng CS, Pal D, Tuzon CT, Schweitzer R, Merrill AE
Development (Cambridge, England) 2019 Aug 2;146(15)
Development (Cambridge, England) 2019 Aug 2;146(15)
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, Magnus T, Stadelmann C, Sirko S, Koch-Nolte F, Eulenburg V, Nicke A
eLife 2018 Aug 3;7
eLife 2018 Aug 3;7
Bioinspired seeding of biomaterials using three dimensional microtissues induces chondrogenic stem cell differentiation and cartilage formation under growth factor free conditions.
Leijten J, Teixeira LS, Bolander J, Ji W, Vanspauwen B, Lammertyn J, Schrooten J, Luyten FP
Scientific reports 2016 Nov 3;6:36011
Scientific reports 2016 Nov 3;6:36011
Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury.
Chen YH, Chen HL, Chien CS, Wu SH, Ho YT, Yu CH, Chang MH
PloS one 2015;10(8):e0134327
PloS one 2015;10(8):e0134327
Porcine SOX9 Gene Expression Is Influenced by an 18 bp Indel in the 5'-Untranslated Region.
Brenig B, Duan Y, Xing Y, Ding N, Huang L, Schütz E
PloS one 2015;10(10):e0139583
PloS one 2015;10(10):e0139583
Prognostic Significance of β-Catenin, E-Cadherin, and SOX9 in Colorectal Cancer: Results from a Large Population-Representative Series.
Bruun J, Kolberg M, Nesland JM, Svindland A, Nesbakken A, Lothe RA
Frontiers in oncology 2014;4:118
Frontiers in oncology 2014;4:118
Sox9 mediates Notch1-induced mesenchymal features in lung adenocarcinoma.
Capaccione KM, Hong X, Morgan KM, Liu W, Bishop JM, Liu L, Markert E, Deen M, Minerowicz C, Bertino JR, Allen T, Pine SR
Oncotarget 2014 Jun 15;5(11):3636-50
Oncotarget 2014 Jun 15;5(11):3636-50
Cell differentiation versus cell death: extracellular glucose is a key determinant of cell fate following oxidative stress exposure.
Poulsen RC, Knowles HJ, Carr AJ, Hulley PA
Cell death & disease 2014 Feb 20;5:e1074
Cell death & disease 2014 Feb 20;5:e1074
Evaluation of protein biomarkers of prostate cancer aggressiveness.
Rizzardi AE, Rosener NK, Koopmeiners JS, Isaksson Vogel R, Metzger GJ, Forster CL, Marston LO, Tiffany JR, McCarthy JB, Turley EA, Warlick CA, Henriksen JC, Schmechel SC
BMC cancer 2014 Apr 5;14:244
BMC cancer 2014 Apr 5;14:244
Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver.
Vestentoft PS, Jelnes P, Hopkinson BM, Vainer B, Møllgård K, Quistorff B, Bisgaard HC
BMC developmental biology 2011 Sep 26;11:56
BMC developmental biology 2011 Sep 26;11:56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SOX9 Antibody [NBP1-85551] - Simple Western lane view shows a specific band for SOX9 in 0.1 mg/ml of U-251MG sp (left) and HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SOX9 Antibody [NBP1-85551] - Electropherogram image(s) of corresponding Simple Western lane view. SOX9 antibody was used at 1:100 dilution on U-251MG sp and HepG2 lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody [NBP1-85551] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody [NBP1-85551] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SOX9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOX9 Antibody [NBP1-85551] - Analysis in control (vector only transfected HEK293T lysate) and SOX9 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424779).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human small intestine shows moderate nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining in human testis and skeletal muscle tissues . Corresponding SOX9 RNA-seq data are presented for the same tissues.
