AF2418

antibody from Novus Biologicals

Targeting: OLIG2 BHLHB1, bHLHe19, OLIGO2, PRKCBP2, RACK17

Provider product page for AF2418

Western blot

Immunocytochemistry

Immunohistochemistry

Chromatin Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [43]
Comments [0]
Validations
Immunohistochemistry [1]
Chromatin Immunoprecipitation [1]

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Product number: AF2418 - Provider product page
Provider: Novus Biologicals
Product name: Goat Polyclonal Olig2 Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.
Reactivity: Human, Mouse, Rat
Host: Goat
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

OLIG2 protein structure - AF2418 shown in red.

Submitted references Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease.

Zhou Y, Song WM, Andhey PS, Swain A, Levy T, Miller KR, Poliani PL, Cominelli M, Grover S, Gilfillan S, Cella M, Ulland TK, Zaitsev K, Miyashita A, Ikeuchi T, Sainouchi M, Kakita A, Bennett DA, Schneider JA, Nichols MR, Beausoleil SA, Ulrich JD, Holtzman DM, Artyomov MN, Colonna M
Nature medicine 2020 Jan;26(1):131-142

Follistatin expression in the central nervous system of the adult rat.

Ogawa C, Mikawa S, Yamashita K, Hayashi Y, Masumoto K, Katou F, Sato K
Journal of chemical neuroanatomy 2020 Apr;105:101753

Comparing modes of delivery of a combination of ion channel inhibitors for limiting secondary degeneration following partial optic nerve transection.

Toomey LM, Bartlett CA, Gavriel N, McGonigle T, Majimbi M, Gopalasingam G, Rodger J, Fitzgerald M
Scientific reports 2019 Oct 25;9(1):15297

Cortical astrocytes develop in a plastic manner at both clonal and cellular levels.

Clavreul S, Abdeladim L, Hernández-Garzón E, Niculescu D, Durand J, Ieng SH, Barry R, Bonvento G, Beaurepaire E, Livet J, Loulier K
Nature communications 2019 Oct 25;10(1):4884

BMP6 expression in the adult rat central nervous system.

Hayashi Y, Mikawa S, Ogawa C, Masumoto K, Katou F, Sato K
Journal of chemical neuroanatomy 2019 Jul;98:41-54

Chronic disturbance in the thalamus following cranial irradiation to the developing mouse brain.

Boström M, Eriksson Y, Danial J, Björk-Eriksson T, Kalm M
Scientific reports 2019 Jul 3;9(1):9588

Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice.

Cathomas F, Azzinnari D, Bergamini G, Sigrist H, Buerge M, Hoop V, Wicki B, Goetze L, Soares S, Kukelova D, Seifritz E, Goebbels S, Nave KA, Ghandour MS, Seoighe C, Hildebrandt T, Leparc G, Klein H, Stupka E, Hengerer B, Pryce CR
Genes, brain, and behavior 2019 Jan;18(1):e12475

Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.

Bellver-Landete V, Bretheau F, Mailhot B, Vallières N, Lessard M, Janelle ME, Vernoux N, Tremblay MÈ, Fuehrmann T, Shoichet MS, Lacroix S
Nature communications 2019 Jan 31;10(1):518

Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation.

Osipovitch M, Asenjo Martinez A, Mariani JN, Cornwell A, Dhaliwal S, Zou L, Chandler-Militello D, Wang S, Li X, Benraiss SJ, Agate R, Lampp A, Benraiss A, Windrem MS, Goldman SA
Cell stem cell 2019 Jan 3;24(1):107-122.e7

Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate.

Chen Q, Wu J, Ye Q, Ma F, Zhu Q, Wu Y, Shan C, Xie X, Li D, Zhan X, Li C, Li XF, Qin X, Zhao T, Wu H, Shi PY, Man J, Qin CF
mBio 2018 Sep 18;9(5)

NF-κB p65 directs sex-specific neuroprotection in human neurons.

Ruiz-Perera LM, Schneider L, Windmöller BA, Müller J, Greiner JFW, Kaltschmidt C, Kaltschmidt B
Scientific reports 2018 Oct 30;8(1):16012

A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors.

Srivastava T, Diba P, Dean JM, Banine F, Shaver D, Hagen M, Gong X, Su W, Emery B, Marks DL, Harris EN, Baggenstoss B, Weigel PH, Sherman LS, Back SA
The Journal of clinical investigation 2018 May 1;128(5):2025-2041

Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.

Datta M, Staszewski O, Raschi E, Frosch M, Hagemeyer N, Tay TL, Blank T, Kreutzfeldt M, Merkler D, Ziegler-Waldkirch S, Matthias P, Meyer-Luehmann M, Prinz M
Immunity 2018 Mar 20;48(3):514-529.e6

Myostatin expression in the adult rat central nervous system.

Hayashi Y, Mikawa S, Ogawa C, Masumoto K, Katou F, Sato K
Journal of chemical neuroanatomy 2018 Dec;94:125-138

Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis.

Falcão AM, van Bruggen D, Marques S, Meijer M, Jäkel S, Agirre E, Samudyata, Floriddia EM, Vanichkina DP, Ffrench-Constant C, Williams A, Guerreiro-Cacais AO, Castelo-Branco G
Nature medicine 2018 Dec;24(12):1837-1844

Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy.

Zhou W, Chen C, Shi Y, Wu Q, Gimple RC, Fang X, Huang Z, Zhai K, Ke SQ, Ping YF, Feng H, Rich JN, Yu JS, Bao S, Bian XW
Cell stem cell 2017 Nov 2;21(5):591-603.e4

The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1.

Young FI, Keruzore M, Nan X, Gennet N, Bellefroid EJ, Li M
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 11;114(28):E5599-E5607

The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation.

Maglorius Renkilaraj MRL, Baudouin L, Wells CM, Doulazmi M, Wehrlé R, Cannaya V, Bachelin C, Barnier JV, Jia Z, Nait Oumesmar B, Dusart I, Bouslama-Oueghlani L
Neurobiology of disease 2017 Feb;98:137-148

Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors.

Yates NJ, Giacci MK, O'Hare Doig RL, Chiha W, Ashworth BE, Kenna J, Bartlett CA, Fitzgerald M
Neural regeneration research 2017 Feb;12(2):307-316

Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors.

Choi SH, Kim SH, Shim KW, Han JW, Choi J, Kim DS, Lyu CJ, Kim JW, Suh CO, Cho J
PloS one 2016;11(4):e0153443

Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression.

Hwang SM, Uhm TG, Lee SK, Kong SK, Jung KH, Binas B, Chai YG, Park SW, Chung IY
Journal of leukocyte biology 2016 Oct;100(4):711-723

RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation.

Xie Q, Wu Q, Kim L, Miller TE, Liau BB, Mack SC, Yang K, Factor DC, Fang X, Huang Z, Zhou W, Alazem K, Wang X, Bernstein BE, Bao S, Rich JN
The Journal of clinical investigation 2016 Jul 1;126(7):2757-72

Brain tissue banking for stem cells for our future.

Palmero E, Palmero S, Murrell W
Scientific reports 2016 Dec 19;6:39394

Patched1 and Patched2 inhibit Smoothened non-cell autonomously.

Roberts B, Casillas C, Alfaro AC, Jägers C, Roelink H
eLife 2016 Aug 23;5

A cord blood monocyte-derived cell therapy product accelerates brain remyelination.

Saha A, Buntz S, Scotland P, Xu L, Noeldner P, Patel S, Wollish A, Gunaratne A, Gentry T, Troy J, Matsushima GK, Kurtzberg J, Balber AE
JCI insight 2016 Aug 18;1(13):e86667

Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro.

Mendes FA, Coelho Aguiar JM, Kahn SA, Reis AH, Dubois LG, Romão LF, Ferreira LS, Chneiweiss H, Moura Neto V, Abreu JG
PloS one 2015;10(8):e0133689

A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.

Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent MF, Veiga-da-Cunha M, Van Schaftingen E
PloS one 2015;10(3):e0119540

Notch signaling regulates motor neuron differentiation of human embryonic stem cells.

Ben-Shushan E, Feldman E, Reubinoff BE
Stem cells (Dayton, Ohio) 2015 Feb;33(2):403-15

Mitochondrial control by DRP1 in brain tumor initiating cells.

Xie Q, Wu Q, Horbinski CM, Flavahan WA, Yang K, Zhou W, Dombrowski SM, Huang Z, Fang X, Shi Y, Ferguson AN, Kashatus DF, Bao S, Rich JN
Nature neuroscience 2015 Apr;18(4):501-10

Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum.

Seto Y, Nakatani T, Masuyama N, Taya S, Kumai M, Minaki Y, Hamaguchi A, Inoue YU, Inoue T, Miyashita S, Fujiyama T, Yamada M, Chapman H, Campbell K, Magnuson MA, Wright CV, Kawaguchi Y, Ikenaka K, Takebayashi H, Ishiwata S, Ono Y, Hoshino M
Nature communications 2014;5:3337

Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells.

Bergsland M, Covacu R, Perez Estrada C, Svensson M, Brundin L
Stem cells (Dayton, Ohio) 2014 Sep;32(9):2539-49

Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.

Yan K, Wu Q, Yan DH, Lee CH, Rahim N, Tritschler I, DeVecchio J, Kalady MF, Hjelmeland AB, Rich JN
Genes & development 2014 May 15;28(10):1085-100

BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells.

Srikanth M, Kim J, Das S, Kessler JA
Molecular cancer research : MCR 2014 Feb;12(2):283-94

Phage display discovery of novel molecular targets in glioblastoma-initiating cells.

Liu JK, Lubelski D, Schonberg DL, Wu Q, Hale JS, Flavahan WA, Mulkearns-Hubert EE, Man J, Hjelmeland AB, Yu J, Lathia JD, Rich JN
Cell death and differentiation 2014 Aug;21(8):1325-39

Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy.

Piltti KM, Salazar DL, Uchida N, Cummings BJ, Anderson AJ
Stem cells translational medicine 2013 Mar;2(3):204-16

The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.

Zelinka CP, Scott MA, Volkov L, Fischer AJ
PloS one 2012;7(9):e44477

N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells.

Martínez-Cerdeño V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS
PloS one 2012;7(6):e39456

Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination.

Kuboyama K, Fujikawa A, Masumura M, Suzuki R, Matsumoto M, Noda M
PloS one 2012;7(11):e48797

Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model.

Salazar DL, Uchida N, Hamers FP, Cummings BJ, Anderson AJ
PloS one 2010 Aug 18;5(8):e12272

A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis.

Weber K, Bartsch U, Stocking C, Fehse B
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Apr;16(4):698-706

Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice.

Widestrand A, Faijerson J, Wilhelmsson U, Smith PL, Li L, Sihlbom C, Eriksson PS, Pekny M
Stem cells (Dayton, Ohio) 2007 Oct;25(10):2619-27

Characterization of progenitor domains in the developing mouse thalamus.

Vue TY, Aaker J, Taniguchi A, Kazemzadeh C, Skidmore JM, Martin DM, Martin JF, Treier M, Nakagawa Y
The Journal of comparative neurology 2007 Nov 1;505(1):73-91

Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain.

Bauer S, Patterson PH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 15;26(46):12089-99

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Olig2 in Mouse Embryo. Olig2 in Mouse Embryo.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation. Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation.