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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX102572 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102572, RRID:AB_1240875
- Product name
- GABPA antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX102572 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABPA antibody detects GABPA protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody (GTX102572) diluted at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 £gg of GABPA antibody [N2C2], Internal (GTX102572).Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
