GTX25079

antibody from GeneTex

Targeting: FOXC1 ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX25079

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25079, RRID:AB_368058

Product name

FOXC1 antibody, C-term

Antibody type

Polyclonal

Reactivity

Human

Host

Goat

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX25079 staining (0.5?g/ml) of Human Bone Marrow lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with GTX25079 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with GTX25079 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX25079 (4?g/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX25079 (3?g/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.