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LearnPA5-18171
antibody from Invitrogen Antibodies
Targeting: FOXC1
ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-18171 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Bone Marrow lysate using Product # PA5-18171 at a concentration of 0.5 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXC1 in HEK293 lysate (10 µg protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 antibody (Product # PA5-18171, 0.5 µg/mL) (Lane A) and probed with anti- DYKDDDDK Tag (1/3000) (Lane C). Mock-transfected HEK293 probed with FOXC1 antibody (Product # PA5-18171, 0.5 mg/mL) (Lane B). Primary incubations were for 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXC1 by a FOXC1 monoclonal antibody (Product # PA5-18171) at a concentration of 0.3 µg/mL. HEK293 (A) Nuclear MCF7 (B) and Negative control Daudi (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FOXC1 in U2OS cells using a FOXC1 polyclonal antibody (Product # PA5-18171) at a concentration of 10 µg/mL. The sample was paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation for 1hr was followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of FOXC1 in HEK293 cells using a FOXC1 monoclonal antibody (Product # PA5-18171) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of FOXC1 in HEK293 cells using a polyclonal antibody (Product #PA5-18171). HEK293 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
