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- Antigen structure
- References [41]
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- Immunohistochemistry [3]
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- Product number
- NBP1-77299 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77299, RRID:AB_11040928
- Product name
- Rabbit Polyclonal RIPK3/RIP3 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Mouse RIPK3/RIP3 Antibody has one isoform (486aa, 53 kDa). Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57 kDa), isoform 2 (252aa, 28 kDa) and isoform 3 (231aa, 25 kDa). Rat RIP3 also has one isoform (478aa, 52 kDa). NBP1-77299 can detect can detect isoforms of mouse and rat as well as human isoform 1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing.
Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis.
Mitochondrial Damage and Necroptosis in Aging Cochlea.
The Neuroprotective Effects of Necrostatin-1 on Subarachnoid Hemorrhage in Rats Are Possibly Mediated by Preventing Blood-Brain Barrier Disruption and RIP3-Mediated Necroptosis.
O-GlcNAc Transferase Suppresses Inflammation and Necroptosis by Targeting Receptor-Interacting Serine/Threonine-Protein Kinase 3.
Central nervous system regeneration is driven by microglia necroptosis and repopulation.
Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.
Blockade of TNF receptor superfamily 1 (TNFR1)-dependent and TNFR1-independent cell death is crucial for normal epidermal differentiation.
Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1.
Post-ischemic salubrinal administration reduces necroptosis in a rat model of global cerebral ischemia.
Neonatal obstructive nephropathy induces necroptosis and necroinflammation.
RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity.
Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis.
Caspase-2 is a negative regulator of necroptosis.
Inhibiting of RIPK3 attenuates early brain injury following subarachnoid hemorrhage: Possibly through alleviating necroptosis.
Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis.
The role of RIP3 in cardiomyocyte necrosis induced by mitochondrial damage of myocardial ischemia-reperfusion.
Sorafenib inhibits therapeutic induction of necroptosis in acute leukemia cells.
Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury.
Nec-1 alleviates cognitive impairment with reduction of Aβ and tau abnormalities in APP/PS1 mice.
Murine cytomegalovirus IE3-dependent transcription is required for DAI/ZBP1-mediated necroptosis.
ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences.
Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium.
Cellular and Molecular Mechanisms Underpinning Macrophage Activation during Remyelination.
MicroRNA-223-5p and -3p Cooperatively Suppress Necroptosis in Ischemic/Reperfused Hearts.
Short form FLICE-inhibitory protein promotes TNFα-induced necroptosis in fibroblasts derived from CFLARs transgenic mice.
CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3.
Intracellular nicotinamide adenine dinucleotide promotes TNF-induced necroptosis in a sirtuin-dependent manner.
Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it.
Receptor interacting protein kinase 1 mediates murine acetaminophen toxicity independent of the necrosome and not through necroptosis.
Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells.
RIPK1 both positively and negatively regulates RIPK3 oligomerization and necroptosis.
RIPK1 blocks early postnatal lethality mediated by caspase-8 and RIPK3.
Cutting Edge: RIP1 kinase activity is dispensable for normal development but is a key regulator of inflammation in SHARPIN-deficient mice.
A positive feedback loop between RIP3 and JNK controls non-alcoholic steatohepatitis.
Survival function of the FADD-CASPASE-8-cFLIP(L) complex.
c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis.
Catalytic activity of the caspase-8-FLIP(L) complex inhibits RIPK3-dependent necrosis.
BNip3 is a mediator of TNF-induced necrotic cell death.
Receptor-interacting protein homotypic interaction motif-dependent control of NF-kappa B activation via the DNA-dependent activator of IFN regulatory factors.
Zhou H, Xu C, Lee H, Yoon Y, Chen W
Molecular metabolism 2020 Jun;36:100971
Molecular metabolism 2020 Jun;36:100971
Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis.
Colijn S, Muthukumar V, Xie J, Gao S, Griffin CT
Disease models & mechanisms 2020 Jan 24;13(1)
Disease models & mechanisms 2020 Jan 24;13(1)
Mitochondrial Damage and Necroptosis in Aging Cochlea.
Lyu AR, Kim TH, Park SJ, Shin SA, Jeong SH, Yu Y, Huh YH, Je AR, Park MJ, Park YH
International journal of molecular sciences 2020 Apr 3;21(7)
International journal of molecular sciences 2020 Apr 3;21(7)
The Neuroprotective Effects of Necrostatin-1 on Subarachnoid Hemorrhage in Rats Are Possibly Mediated by Preventing Blood-Brain Barrier Disruption and RIP3-Mediated Necroptosis.
Chen J, Jin H, Xu H, Peng Y, Jie L, Xu D, Chen L, Li T, Fan L, He P, Ying G, Gu C, Wang C, Wang L, Chen G
Cell transplantation 2019 Nov;28(11):1358-1372
Cell transplantation 2019 Nov;28(11):1358-1372
O-GlcNAc Transferase Suppresses Inflammation and Necroptosis by Targeting Receptor-Interacting Serine/Threonine-Protein Kinase 3.
Li X, Gong W, Wang H, Li T, Attri KS, Lewis RE, Kalil AC, Bhinderwala F, Powers R, Yin G, Herring LE, Asara JM, Lei YL, Yang X, Rodriguez DA, Yang M, Green DR, Singh PK, Wen H
Immunity 2019 Mar 19;50(3):576-590.e6
Immunity 2019 Mar 19;50(3):576-590.e6
Central nervous system regeneration is driven by microglia necroptosis and repopulation.
Lloyd AF, Davies CL, Holloway RK, Labrak Y, Ireland G, Carradori D, Dillenburg A, Borger E, Soong D, Richardson JC, Kuhlmann T, Williams A, Pollard JW, des Rieux A, Priller J, Miron VE
Nature neuroscience 2019 Jul;22(7):1046-1052
Nature neuroscience 2019 Jul;22(7):1046-1052
Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.
Lim J, Park H, Heisler J, Maculins T, Roose-Girma M, Xu M, Mckenzie B, van Lookeren Campagne M, Newton K, Murthy A
eLife 2019 Jul 9;8
eLife 2019 Jul 9;8
Blockade of TNF receptor superfamily 1 (TNFR1)-dependent and TNFR1-independent cell death is crucial for normal epidermal differentiation.
Piao X, Miura R, Miyake S, Komazawa-Sakon S, Koike M, Shindo R, Takeda J, Hasegawa A, Abe R, Nishiyama C, Mikami T, Yagita H, Uchiyama Y, Nakano H
The Journal of allergy and clinical immunology 2019 Jan;143(1):213-228.e10
The Journal of allergy and clinical immunology 2019 Jan;143(1):213-228.e10
Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1.
Du XK, Ge WY, Jing R, Pan LH
International immunopharmacology 2019 Dec;77:105944
International immunopharmacology 2019 Dec;77:105944
Post-ischemic salubrinal administration reduces necroptosis in a rat model of global cerebral ischemia.
Font-Belmonte E, Ugidos IF, Santos-Galdiano M, González-Rodríguez P, Anuncibay-Soto B, Pérez-Rodríguez D, Gonzalo-Orden JM, Fernández-López A
Journal of neurochemistry 2019 Dec;151(6):777-794
Journal of neurochemistry 2019 Dec;151(6):777-794
Neonatal obstructive nephropathy induces necroptosis and necroinflammation.
Popper B, Rammer MT, Gasparitsch M, Singer T, Keller U, Döring Y, Lange-Sperandio B
Scientific reports 2019 Dec 9;9(1):18600
Scientific reports 2019 Dec 9;9(1):18600
RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity.
Orozco SL, Daniels BP, Yatim N, Messmer MN, Quarato G, Chen-Harris H, Cullen SP, Snyder AG, Ralli-Jain P, Frase S, Tait SWG, Green DR, Albert ML, Oberst A
Cell reports 2019 Aug 27;28(9):2275-2287.e5
Cell reports 2019 Aug 27;28(9):2275-2287.e5
Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis.
Rodriguez DA, Green DR
Methods in molecular biology (Clifton, N.J.) 2018;1857:71-83
Methods in molecular biology (Clifton, N.J.) 2018;1857:71-83
Caspase-2 is a negative regulator of necroptosis.
Zamaraev AV, Kopeina GS, Buchbinder JH, Zhivotovsky B, Lavrik IN
The international journal of biochemistry & cell biology 2018 Sep;102:101-108
The international journal of biochemistry & cell biology 2018 Sep;102:101-108
Inhibiting of RIPK3 attenuates early brain injury following subarachnoid hemorrhage: Possibly through alleviating necroptosis.
Chen T, Pan H, Li J, Xu H, Jin H, Qian C, Yan F, Chen J, Wang C, Chen J, Wang L, Chen G
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Nov;107:563-570
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Nov;107:563-570
Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis.
Li P, Zhou Y, Goodwin AJ, Cook JA, Halushka PV, Zhang XK, Wilson CL, Schnapp LM, Zingarelli B, Fan H
The Journal of infectious diseases 2018 Nov 5;218(12):1995-2005
The Journal of infectious diseases 2018 Nov 5;218(12):1995-2005
The role of RIP3 in cardiomyocyte necrosis induced by mitochondrial damage of myocardial ischemia-reperfusion.
Hou H, Wang Y, Li Q, Li Z, Teng Y, Li J, Wang X, Chen J, Huang N
Acta biochimica et biophysica Sinica 2018 Nov 1;50(11):1131-1140
Acta biochimica et biophysica Sinica 2018 Nov 1;50(11):1131-1140
Sorafenib inhibits therapeutic induction of necroptosis in acute leukemia cells.
Feldmann F, Schenk B, Martens S, Vandenabeele P, Fulda S
Oncotarget 2017 Sep 15;8(40):68208-68220
Oncotarget 2017 Sep 15;8(40):68208-68220
Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury.
Martens S, Jeong M, Tonnus W, Feldmann F, Hofmans S, Goossens V, Takahashi N, Bräsen JH, Lee EW, Van der Veken P, Joossens J, Augustyns K, Fulda S, Linkermann A, Song J, Vandenabeele P
Cell death & disease 2017 Jun 29;8(6):e2904
Cell death & disease 2017 Jun 29;8(6):e2904
Nec-1 alleviates cognitive impairment with reduction of Aβ and tau abnormalities in APP/PS1 mice.
Yang SH, Lee DK, Shin J, Lee S, Baek S, Kim J, Jung H, Hah JM, Kim Y
EMBO molecular medicine 2017 Jan;9(1):61-77
EMBO molecular medicine 2017 Jan;9(1):61-77
Murine cytomegalovirus IE3-dependent transcription is required for DAI/ZBP1-mediated necroptosis.
Sridharan H, Ragan KB, Guo H, Gilley RP, Landsteiner VJ, Kaiser WJ, Upton JW
EMBO reports 2017 Aug;18(8):1429-1441
EMBO reports 2017 Aug;18(8):1429-1441
ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences.
Gong YN, Guy C, Olauson H, Becker JU, Yang M, Fitzgerald P, Linkermann A, Green DR
Cell 2017 Apr 6;169(2):286-300.e16
Cell 2017 Apr 6;169(2):286-300.e16
Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium.
Ros U, Peña-Blanco A, Hänggi K, Kunzendorf U, Krautwald S, Wong WW, García-Sáez AJ
Cell reports 2017 Apr 4;19(1):175-187
Cell reports 2017 Apr 4;19(1):175-187
Cellular and Molecular Mechanisms Underpinning Macrophage Activation during Remyelination.
Lloyd AF, Miron VE
Frontiers in cell and developmental biology 2016;4:60
Frontiers in cell and developmental biology 2016;4:60
MicroRNA-223-5p and -3p Cooperatively Suppress Necroptosis in Ischemic/Reperfused Hearts.
Qin D, Wang X, Li Y, Yang L, Wang R, Peng J, Essandoh K, Mu X, Peng T, Han Q, Yu KJ, Fan GC
The Journal of biological chemistry 2016 Sep 16;291(38):20247-59
The Journal of biological chemistry 2016 Sep 16;291(38):20247-59
Short form FLICE-inhibitory protein promotes TNFα-induced necroptosis in fibroblasts derived from CFLARs transgenic mice.
Shindo R, Yamazaki S, Ohmuraya M, Araki K, Nakano H
Biochemical and biophysical research communications 2016 Nov 4;480(1):23-28
Biochemical and biophysical research communications 2016 Nov 4;480(1):23-28
CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3.
Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
Nature cell biology 2016 Mar;18(3):291-302
Nature cell biology 2016 Mar;18(3):291-302
Intracellular nicotinamide adenine dinucleotide promotes TNF-induced necroptosis in a sirtuin-dependent manner.
Preyat N, Rossi M, Kers J, Chen L, Bertin J, Gough PJ, Le Moine A, Rongvaux A, Van Gool F, Leo O
Cell death and differentiation 2016 Jan;23(1):29-40
Cell death and differentiation 2016 Jan;23(1):29-40
Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it.
Yoon S, Bogdanov K, Kovalenko A, Wallach D
Cell death and differentiation 2016 Feb;23(2):253-60
Cell death and differentiation 2016 Feb;23(2):253-60
Receptor interacting protein kinase 1 mediates murine acetaminophen toxicity independent of the necrosome and not through necroptosis.
Dara L, Johnson H, Suda J, Win S, Gaarde W, Han D, Kaplowitz N
Hepatology (Baltimore, Md.) 2015 Dec;62(6):1847-57
Hepatology (Baltimore, Md.) 2015 Dec;62(6):1847-57
Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
Wertz IE, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman RJ, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother WJ, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee WP, Goodnow CC, Balazs M, Yu K, Kolumam G, Dixit VM
Nature 2015 Dec 17;528(7582):370-5
Nature 2015 Dec 17;528(7582):370-5
Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells.
Miki Y, Akimoto J, Moritake K, Hironaka C, Fujiwara Y
Lasers in medical science 2015 Aug;30(6):1739-45
Lasers in medical science 2015 Aug;30(6):1739-45
RIPK1 both positively and negatively regulates RIPK3 oligomerization and necroptosis.
Orozco S, Yatim N, Werner MR, Tran H, Gunja SY, Tait SW, Albert ML, Green DR, Oberst A
Cell death and differentiation 2014 Oct;21(10):1511-21
Cell death and differentiation 2014 Oct;21(10):1511-21
RIPK1 blocks early postnatal lethality mediated by caspase-8 and RIPK3.
Dillon CP, Weinlich R, Rodriguez DA, Cripps JG, Quarato G, Gurung P, Verbist KC, Brewer TL, Llambi F, Gong YN, Janke LJ, Kelliher MA, Kanneganti TD, Green DR
Cell 2014 May 22;157(5):1189-202
Cell 2014 May 22;157(5):1189-202
Cutting Edge: RIP1 kinase activity is dispensable for normal development but is a key regulator of inflammation in SHARPIN-deficient mice.
Berger SB, Kasparcova V, Hoffman S, Swift B, Dare L, Schaeffer M, Capriotti C, Cook M, Finger J, Hughes-Earle A, Harris PA, Kaiser WJ, Mocarski ES, Bertin J, Gough PJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5476-80
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5476-80
A positive feedback loop between RIP3 and JNK controls non-alcoholic steatohepatitis.
Gautheron J, Vucur M, Reisinger F, Cardenas DV, Roderburg C, Koppe C, Kreggenwinkel K, Schneider AT, Bartneck M, Neumann UP, Canbay A, Reeves HL, Luedde M, Tacke F, Trautwein C, Heikenwalder M, Luedde T
EMBO molecular medicine 2014 Aug;6(8):1062-74
EMBO molecular medicine 2014 Aug;6(8):1062-74
Survival function of the FADD-CASPASE-8-cFLIP(L) complex.
Dillon CP, Oberst A, Weinlich R, Janke LJ, Kang TB, Ben-Moshe T, Mak TW, Wallach D, Green DR
Cell reports 2012 May 31;1(5):401-7
Cell reports 2012 May 31;1(5):401-7
c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis.
Piao X, Komazawa-Sakon S, Nishina T, Koike M, Piao JH, Ehlken H, Kurihara H, Hara M, Van Rooijen N, Schütz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, Nakano H
Science signaling 2012 Dec 18;5(255):ra93
Science signaling 2012 Dec 18;5(255):ra93
Catalytic activity of the caspase-8-FLIP(L) complex inhibits RIPK3-dependent necrosis.
Oberst A, Dillon CP, Weinlich R, McCormick LL, Fitzgerald P, Pop C, Hakem R, Salvesen GS, Green DR
Nature 2011 Mar 17;471(7338):363-7
Nature 2011 Mar 17;471(7338):363-7
BNip3 is a mediator of TNF-induced necrotic cell death.
Kim JY, Kim YJ, Lee S, Park JH
Apoptosis : an international journal on programmed cell death 2011 Feb;16(2):114-26
Apoptosis : an international journal on programmed cell death 2011 Feb;16(2):114-26
Receptor-interacting protein homotypic interaction motif-dependent control of NF-kappa B activation via the DNA-dependent activator of IFN regulatory factors.
Kaiser WJ, Upton JW, Mocarski ES
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6427-34
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6427-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Western Blot Validation in Human Cell Lines. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed molecular weight ~ 57 kDa. Theoretical molecular weight 53 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Western Blot Validation in C2C12 Cells. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: NBP1-77299, 0.1 ug/mL in the presence of peptide blocking. Lane 2: NBP1-77299, 0.1 ug/mL Lane 3: NBP1-77299, 0.2 ug/mL Lane 4: NBP1-77299, 0.5 ug/mL. Observed molecular weight ~55 kDa. Theoretical molecular weight 53 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Western Blot Validation in Mouse Cell lines. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 Antibody [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed molecular weight ~50 kDa. Theoretical molecular weight 53 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis using RIPK3/RIP3 antibody [NBP1-77299]. L929 cell line (mouse). 20 ug whole cell lysate. Primary antibody: 1:2000. Secondary antibody: 1:20000. Western blot image submitted by a verified customer review. Observed molecular weight ~57 kDa. Theoretical molecular weight 53 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Rat kidney tissue with RIPK3/RIP3 Antibody [NBP1-77299] at 5 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RIPK3/RIP3 Antibody [NBP1-77299] - IHC analysis of a murine kidney tissue section using RIP3 antibody at 20 ug/mL concentration and IF based detection.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Immunohistochemistry analysis of a paraffin embedded mouse kidney tissue section using RIPK3/RIP3 Antibody [NBP1-77299] at 2.5 ug/mL concentration.
