Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-24588 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RIPK3/RIP3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Biomarker profile for prediction of response to SMAC mimetic monotherapy in pediatric precursor B-cell acute lymphoblastic leukemia.
TAK1 regulates endothelial cell necroptosis and tumor metastasis.
DPS-2: A Novel Dual MEK/ERK and PI3K/AKT Pathway Inhibitor with Powerful Ex Vivo and In Vivo Anticancer Properties.
TAK1 suppresses RIPK1-dependent cell death and is associated with disease progression in melanoma.
RIP1, RIP3, and MLKL Contribute to Cell Death Caused by Clostridium perfringens Enterotoxin.
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death.
MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D.
Coordinated ubiquitination and phosphorylation of RIP1 regulates necroptotic cell death.
RSL3 and Erastin differentially regulate redox signaling to promote Smac mimetic-induced cell death.
Molecular features of the cytotoxicity of an NHE inhibitor: Evidence of mitochondrial alterations, ROS overproduction and DNA damage.
Discovery of piperlongumine as a potential novel lead for the development of senolytic agents.
Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-α-dependent necroptosis driven by RIP1 kinase and NF-κB.
IAP antagonists Birinapant and AT-406 efficiently synergise with either TRAIL, BRAF, or BCL-2 inhibitors to sensitise BRAFV600E colorectal tumour cells to apoptosis.
TRAF2 inhibits TRAIL- and CD95L-induced apoptosis and necroptosis.
A positive feedback loop between RIP3 and JNK controls non-alcoholic steatohepatitis.
Vaccinia virus induces programmed necrosis in ovarian cancer cells.
Intrinsic cleavage of receptor-interacting protein kinase-1 by caspase-6.
Astrovirus MLB1 is not associated with diarrhea in a cohort of Indian children.
Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice.
TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
Nitration of the mitochondrial complex I subunit NDUFB8 elicits RIP1- and RIP3-mediated necrosis.
Zinngrebe J, Schlichtig F, Kraus JM, Meyer M, Boldrin E, Kestler HA, Meyer LH, Fischer-Posovszky P, Debatin KM
International journal of cancer 2020 Jun 1;146(11):3219-3231
International journal of cancer 2020 Jun 1;146(11):3219-3231
TAK1 regulates endothelial cell necroptosis and tumor metastasis.
Yang L, Joseph S, Sun T, Hoffmann J, Thevissen S, Offermanns S, Strilic B
Cell death and differentiation 2019 Oct;26(10):1987-1997
Cell death and differentiation 2019 Oct;26(10):1987-1997
DPS-2: A Novel Dual MEK/ERK and PI3K/AKT Pathway Inhibitor with Powerful Ex Vivo and In Vivo Anticancer Properties.
Goulielmaki M, Assimomytis N, Rozanc J, Taki E, Christodoulou I, Alexopoulos LG, Zoumpourlis V, Pintzas A, Papahatjis D
Translational oncology 2019 Jul;12(7):932-950
Translational oncology 2019 Jul;12(7):932-950
TAK1 suppresses RIPK1-dependent cell death and is associated with disease progression in melanoma.
Podder B, Guttà C, Rožanc J, Gerlach E, Feoktistova M, Panayotova-Dimitrova D, Alexopoulos LG, Leverkus M, Rehm M
Cell death and differentiation 2019 Dec;26(12):2520-2534
Cell death and differentiation 2019 Dec;26(12):2520-2534
RIP1, RIP3, and MLKL Contribute to Cell Death Caused by Clostridium perfringens Enterotoxin.
Shrestha A, Mehdizadeh Gohari I, McClane BA
mBio 2019 Dec 17;10(6)
mBio 2019 Dec 17;10(6)
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death.
Lafont E, Kantari-Mimoun C, Draber P, De Miguel D, Hartwig T, Reichert M, Kupka S, Shimizu Y, Taraborrelli L, Spit M, Sprick MR, Walczak H
The EMBO journal 2017 May 2;36(9):1147-1166
The EMBO journal 2017 May 2;36(9):1147-1166
MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D.
Gutierrez KD, Davis MA, Daniels BP, Olsen TM, Ralli-Jain P, Tait SW, Gale M Jr, Oberst A
Journal of immunology (Baltimore, Md. : 1950) 2017 Mar 1;198(5):2156-2164
Journal of immunology (Baltimore, Md. : 1950) 2017 Mar 1;198(5):2156-2164
Coordinated ubiquitination and phosphorylation of RIP1 regulates necroptotic cell death.
de Almagro MC, Goncharov T, Izrael-Tomasevic A, Duttler S, Kist M, Varfolomeev E, Wu X, Lee WP, Murray J, Webster JD, Yu K, Kirkpatrick DS, Newton K, Vucic D
Cell death and differentiation 2017 Jan;24(1):26-37
Cell death and differentiation 2017 Jan;24(1):26-37
RSL3 and Erastin differentially regulate redox signaling to promote Smac mimetic-induced cell death.
Dächert J, Schoeneberger H, Rohde K, Fulda S
Oncotarget 2016 Sep 27;7(39):63779-63792
Oncotarget 2016 Sep 27;7(39):63779-63792
Molecular features of the cytotoxicity of an NHE inhibitor: Evidence of mitochondrial alterations, ROS overproduction and DNA damage.
Aredia F, Czaplinski S, Fulda S, Scovassi AI
BMC cancer 2016 Nov 5;16(1):851
BMC cancer 2016 Nov 5;16(1):851
Discovery of piperlongumine as a potential novel lead for the development of senolytic agents.
Wang Y, Chang J, Liu X, Zhang X, Zhang S, Zhang X, Zhou D, Zheng G
Aging 2016 Nov 19;8(11):2915-2926
Aging 2016 Nov 19;8(11):2915-2926
Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-α-dependent necroptosis driven by RIP1 kinase and NF-κB.
Oliver Metzig M, Fuchs D, Tagscherer KE, Gröne HJ, Schirmacher P, Roth W
Oncogene 2016 Jun 30;35(26):3399-409
Oncogene 2016 Jun 30;35(26):3399-409
IAP antagonists Birinapant and AT-406 efficiently synergise with either TRAIL, BRAF, or BCL-2 inhibitors to sensitise BRAFV600E colorectal tumour cells to apoptosis.
Perimenis P, Galaris A, Voulgari A, Prassa M, Pintzas A
BMC cancer 2016 Aug 12;16:624
BMC cancer 2016 Aug 12;16:624
TRAF2 inhibits TRAIL- and CD95L-induced apoptosis and necroptosis.
Karl I, Jossberger-Werner M, Schmidt N, Horn S, Goebeler M, Leverkus M, Wajant H, Giner T
Cell death & disease 2014 Oct 9;5:e1444
Cell death & disease 2014 Oct 9;5:e1444
A positive feedback loop between RIP3 and JNK controls non-alcoholic steatohepatitis.
Gautheron J, Vucur M, Reisinger F, Cardenas DV, Roderburg C, Koppe C, Kreggenwinkel K, Schneider AT, Bartneck M, Neumann UP, Canbay A, Reeves HL, Luedde M, Tacke F, Trautwein C, Heikenwalder M, Luedde T
EMBO molecular medicine 2014 Aug;6(8):1062-74
EMBO molecular medicine 2014 Aug;6(8):1062-74
Vaccinia virus induces programmed necrosis in ovarian cancer cells.
Whilding LM, Archibald KM, Kulbe H, Balkwill FR, Öberg D, McNeish IA
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Nov;21(11):2074-86
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Nov;21(11):2074-86
Intrinsic cleavage of receptor-interacting protein kinase-1 by caspase-6.
van Raam BJ, Ehrnhoefer DE, Hayden MR, Salvesen GS
Cell death and differentiation 2013 Jan;20(1):86-96
Cell death and differentiation 2013 Jan;20(1):86-96
Astrovirus MLB1 is not associated with diarrhea in a cohort of Indian children.
Holtz LR, Bauer IK, Rajendran P, Kang G, Wang D
PloS one 2011;6(12):e28647
PloS one 2011;6(12):e28647
Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice.
Csak T, Dolganiuc A, Kodys K, Nath B, Petrasek J, Bala S, Lippai D, Szabo G
Hepatology (Baltimore, Md.) 2011 Jun;53(6):1917-31
Hepatology (Baltimore, Md.) 2011 Jun;53(6):1917-31
TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
Ikner A, Ashkenazi A
The Journal of biological chemistry 2011 Jun 17;286(24):21546-54
The Journal of biological chemistry 2011 Jun 17;286(24):21546-54
Nitration of the mitochondrial complex I subunit NDUFB8 elicits RIP1- and RIP3-mediated necrosis.
Davis CW, Hawkins BJ, Ramasamy S, Irrinki KM, Cameron BA, Islam K, Daswani VP, Doonan PJ, Manevich Y, Madesh M
Free radical biology & medicine 2010 Jan 15;48(2):306-17
Free radical biology & medicine 2010 Jan 15;48(2):306-17
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 antibody [NBP2-24588]. Human tissue blot probed with 5 ug/ml of RIP3 antibody: 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, 9) spleen, 10) ovary, 11) testis, 12) placenta probed at 5 ug/ml, 13) Ramos cell lysate probed at 7 ug/ml. Theoretical molecular weight 56.7 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.
