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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V2712 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MITF Antibody
- Antibody type
- Monoclonal
- Antigen
- An N-terminus fragment of human Mi protein was used as the immunogen for the MITF antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- D5
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the MITF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Nevospheres from neurocutaneous melanocytosis cells show reduced viability when treated with specific inhibitors of NRAS signaling pathway.
Emerging clinical applications of selected biomarkers in melanoma.
Loss of CITED1, an MITF regulator, drives a phenotype switch in vitro and can predict clinical outcome in primary melanoma tumours.
Immunohistochemical profile of oral mucosal and head and neck cutaneous melanoma.
TFE3 translocation-associated perivascular epithelioid cell neoplasm (PEComa) of the gynecologic tract: morphology, immunophenotype, differential diagnosis.
Expression Patterns of VEGF and Flk-1 in Human Endometrium during the Menstrual Cycle.
An unusual case of desmoplastic melanoma containing an osteoclast-like giant cell-rich nodule.
Prevalence and heterogeneity of circulating tumour cells in metastatic cutaneous melanoma.
SOX-10 and MiTF expression in cellular and 'mixed' neurothekeoma.
Basu D, Salgado CM, Bauer BS, Johnson D, Rundell V, Nikiforova M, Khakoo Y, Gunwaldt LJ, Panigrahy A, Reyes-Múgica M
Neuro-oncology 2016 Apr;18(4):528-37
Neuro-oncology 2016 Apr;18(4):528-37
Emerging clinical applications of selected biomarkers in melanoma.
Tetzlaff MT, Torres-Cabala CA, Pattanaprichakul P, Rapini RP, Prieto VG, Curry JL
Clinical, cosmetic and investigational dermatology 2015;8:35-46
Clinical, cosmetic and investigational dermatology 2015;8:35-46
Loss of CITED1, an MITF regulator, drives a phenotype switch in vitro and can predict clinical outcome in primary melanoma tumours.
Howlin J, Cirenajwis H, Lettiero B, Staaf J, Lauss M, Saal L, Borg K, Gruvberger-Saal S, Jönsson G
PeerJ 2015;3:e788
PeerJ 2015;3:e788
Immunohistochemical profile of oral mucosal and head and neck cutaneous melanoma.
Alaeddini M, Etemad-Moghadam S
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2015 Mar;44(3):234-8
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2015 Mar;44(3):234-8
TFE3 translocation-associated perivascular epithelioid cell neoplasm (PEComa) of the gynecologic tract: morphology, immunophenotype, differential diagnosis.
Schoolmeester JK, Dao LN, Sukov WR, Wang L, Park KJ, Murali R, Hameed MR, Soslow RA
The American journal of surgical pathology 2015 Mar;39(3):394-404
The American journal of surgical pathology 2015 Mar;39(3):394-404
Expression Patterns of VEGF and Flk-1 in Human Endometrium during the Menstrual Cycle.
Lai TH, Vlahos N, Shih IeM, Zhao Y
Journal of reproduction & infertility 2015 Jan-Mar;16(1):3-9
Journal of reproduction & infertility 2015 Jan-Mar;16(1):3-9
An unusual case of desmoplastic melanoma containing an osteoclast-like giant cell-rich nodule.
Houang M, Castillo C, La Marca S, Combemale P, Wang Q, Paindavoine S, Pissaloux D, de la Fouchardiere A
The American Journal of dermatopathology 2015 Apr;37(4):299-304
The American Journal of dermatopathology 2015 Apr;37(4):299-304
Prevalence and heterogeneity of circulating tumour cells in metastatic cutaneous melanoma.
Khoja L, Shenjere P, Hodgson C, Hodgetts J, Clack G, Hughes A, Lorigan P, Dive C
Melanoma research 2014 Feb;24(1):40-6
Melanoma research 2014 Feb;24(1):40-6
SOX-10 and MiTF expression in cellular and 'mixed' neurothekeoma.
Fried I, Sitthinamsuwan P, Muangsomboon S, Kaddu S, Cerroni L, McCalmont TH
Journal of cutaneous pathology 2014 Aug;41(8):640-5
Journal of cutaneous pathology 2014 Aug;41(8):640-5
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate (nuclear fraction) with MITF antibody (clone D5). Predicted molecular weight: 55-60kDa, observed here at 50-55kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with MITF antibody (D5).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free MITF Antibody (clone D5). Confirmation of Integrity and Purity of the Antibody.
