Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
- Other assay [1]
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- Product number
- PA5-104707 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MiTF (Ser180, Ser73) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references PPP6C, a serine-threonine phosphatase, regulates melanocyte differentiation and contributes to melanoma tumorigenesis through modulation of MITF activity.
Maskin CR, Raman R, Houvras Y
Scientific reports 2022 Apr 2;12(1):5573
Scientific reports 2022 Apr 2;12(1):5573
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MiTF (Ser180, Ser73) in mouse heart/mouse lung (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-MiTF (Ser180, Ser73) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in human heart tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in human heart tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in mouse lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in rat ovarian tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in human liver tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in rat uterine tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in mouse brain tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-MiTF (Ser180, Ser73) in mouse gastric tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MiTF (Ser180, Ser73) polyclonal antibody (Product # PA5-104707) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 PPP6C expression affects MITF expression and drug resistance in melanoma. ( A ) Real-time PCR measurement of PPP6C in cells 72 h after transfection with either siPPP6C or siNon-targeting (Student's two-tailed t-test). ( B ) Real-time PCR measurement of MITF and three target genes in cells with and without PPP6C knockdown 72 h after transfection (Student's two-tailed t-test). ( C ) Drug dose response curve for A375 cells treated with dabrafenib and knockdown of PPP6C 72 h after transfection and treatment. Dotted lines display EC 50 for each condition. ( D ) Cellular expression of total MITF in A375 cells. Representative confocal images of fluorescence 72 h after transfection with either siPPP6C or siNon-targeting and staining with a MITF antibody, with quantification of fluorescence for each condition. One of two independent experiments is shown (Student's two-tailed t-test). ( E ) Cellular expression of phospho-MITF in A375 cells. Representative confocal images of fluorescence 72 h after transfection with either siPPP6C or siNon-targeting and staining with a phospho-specific MITF antibody, with quantification of fluorescence for each condition. One of two independent experiments is shown (Student's two-tailed t-test). p -values are indicated as following: * p < 0.05, ** p