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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX111131 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111131, RRID:AB_2038000
- Product name
- SMAD2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Fgf9 inhibition of meiotic differentiation in spermatogonia is mediated by Erk-dependent activation of Nodal-Smad2/3 signaling and is antagonized by Kit Ligand.
The transcription factor GLI1 interacts with SMAD proteins to modulate transforming growth factor β-induced gene expression in a p300/CREB-binding protein-associated factor (PCAF)-dependent manner.
Tassinari V, Campolo F, Cesarini V, Todaro F, Dolci S, Rossi P
Cell death & disease 2015 Mar 12;6(3):e1688
Cell death & disease 2015 Mar 12;6(3):e1688
The transcription factor GLI1 interacts with SMAD proteins to modulate transforming growth factor β-induced gene expression in a p300/CREB-binding protein-associated factor (PCAF)-dependent manner.
Nye MD, Almada LL, Fernandez-Barrena MG, Marks DL, Elsawa SF, Vrabel A, Tolosa EJ, Ellenrieder V, Fernandez-Zapico ME
The Journal of biological chemistry 2014 May 30;289(22):15495-506
The Journal of biological chemistry 2014 May 30;289(22):15495-506
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 10% SDS PAGE GTX111131 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2(GTX111131) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 50 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 £gg of SMAD2antibody (GTX111131) 10% SDS-PAGE The immunoprecipitated SMAD2protein was detected by SMAD2antibody (GTX111131) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of SMAD2 antibody (GTX111131) The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.
