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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F50345 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SMAD2 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 97-125 from the human protein was used as the immunogen for this SMAD2 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD2 antibody and NIH3T3 lysate. Predicted molecular weight: 52~60 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody at 1:100. Note the highly specific localization of the SMAD2 to the nucleus.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SMAD2 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
