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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- F48613 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-SMAD2 Antibody (pT220)
- Antibody type
- Polyclonal
- Antigen
- This phospho-SMAD2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT220 of human SMAD2.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the phospho-SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells/lysate, untreated or treated with PMA, using phospho-SMAD2 antibody (left) or nonphos Ab (right).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of phospho-SMAD2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-SMAD2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
