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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AM06653SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM06653SU-N, RRID:AB_11216843
- Product name
- anti SMAD2
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of human SMAD2 expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G7
- Vial size
- 0.1 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using SMAD2 mAb against human SMAD2 (AA: 20-254) recombinant protein. (Expected MW is 52.2 kDa)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U251 cells using SMAD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using SMAD2 mouse mAb with DAB staining.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using SMAD2 mouse mAb (blue) and negative control (red).
