Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 11773-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ATLKAELLS LKLKFGLISS TAYAQEIQKL SNSTAVYFQD YQTSKSNVSS FVDEHEPSMV SSSCISVIKH SPQSSLSDVS EVSSVEHTQE SSVQGSCRSP ENKFQIIKQE PMELESYTRE PRDDRGSYTA SIYQNYMGNS FSGYSHSPPL LQVNRSSSNS PRTSETDDGV VGKSSDGEDE QQVPKGPIHS PVELKHVHAT VVKVPEVNSS ALPHKLRIKA KAMQIKVEAF DNEFEATQKL SSPIDMTSKR HFELEKHSAP SMVHSSLTPF SVQVTNIQDW SLKSEHWHQK ELSGKTQNSF KTGVVEMKDS GYKVSDPENL YLKQGIANLS AEVVSLKRLI ATQPISASDS G (113-462 aa encoded by BC008197)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.41 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 11773-1-AP (NFIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NFIL3 (IP:11773-1-AP, 4ug; Detection:11773-1-AP 1:1000) with HeLa cells lysate 1600ug.