AP6753C

antibody from Abgent

Targeting: SMAD7 MADH7, MADH8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6753C

Provider

Abgent

Proper citation

Abgent Cat#AP6753c, RRID:AB_10615246

Product name

Smad7 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of Smad7 Antibody (Center) (Cat. #AP6753c) in ZR-75-1 cell line lysates (35ug/lane). Smad7 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"""Fluorescent confocal image of A431 cell stained with Smad7 Antibody (Center)(Cat#AP6753c). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).Smad7 immunoreactivity is localized to cytoplasm significantly. "" "

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Smad7 Antibody (Center) (Cat. #AP6753c) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Smad7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50