H00001992-M02

antibody from Abnova Corporation

Targeting: SERPINB1 anti-elastase, EI, ELANH2, PI2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001992-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001992-M02, RRID:AB_1204963

Product name

SERPINB1 monoclonal antibody (M02), clone 4D7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SERPINB1.

Antigen sequence

KKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDD
IEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVN
VSLPRFKLEESYTLNSDLARLGVQDLFNSS


Isotype

IgG

Antibody clone number

4D7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SERPINB1 expression in transfected 293T cell line by SERPINB1 monoclonal antibody (M02), clone 4D7.Lane 1: SERPINB1 transfected lysate(42.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SERPINB1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SERPINB1 on HeLa cell . [antibody concentration 40 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol