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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00063876-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00063876-M01, RRID:AB_547091
- Product name
- Mouse Monoclonal PKNOX2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PKNOX2 - PBX/knotted 1 homeobox 2
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi ( Cat # H00063876-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody (M01), clone 4B6 (Cat # H00063876-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody (M01), clone 4B6.Lane 1: PKNOX2 transfected lysate(51.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - PKNOX2 monoclonal antibody (M01), clone 4B6. Analysis of PKNOX2 expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PKNOX2 Antibody (4B6) [H00063876-M01] - Detection limit for recombinant GST tagged PKNOX2 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of monoclonal antibody to PKNOX2 on HeLa cell. Antibody concentration 10 ug/ml.
