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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- GTX129030 - Provider product page
- Provider
- GeneTex
- Product name
- SP2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- SP2 antibody detects SP2 protein by western blot analysis.A. 30 £gg Jurkat whole cell lysate/extract B. 30 £gg Raji whole cell lysate/extract C. 30 £gg NCI-H929 whole cell lysate/extract7.5 % SDS-PAGESP2 antibody (GTX129030) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SP2 antibody (GTX129030) diluted at 1:1000 and competitor's antibody (SC-643) diluted by 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SP2 antibody immunoprecipitates SP2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 £gg whole cell lysate/extract of SP2 protein expressing Raji cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of SP2 by 3 £gg of SP2 antibody (GTX129030) 5% SDS-PAGE The immunoprecipitated SP2 protein was detected by SP2 antibody (GTX129030) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
