PA5-35984

antibody from Invitrogen Antibodies

Targeting: SP2 KIAA0048

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35984

Provider

Invitrogen Antibodies

Product name

SP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Raji, NCI-H929, A431, HepG2. Predicted reactivity: Mouse (91%), Rat (90%), Rhesus Monkey (100%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.65 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

DUSP5 promotes osteogenic differentiation through SCP1/2-dependent phosphorylation of SMAD1.

Liu X, Liu X, Du Y, Hu M, Tian Y, Li Z, Lv L, Zhang X, Liu Y, Zhou Y, Zhang P
Stem cells (Dayton, Ohio) 2021 Oct;39(10):1395-1409

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SP2 using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate and C) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SP2 polyclonal antibody (Product # PA5-35984) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SP2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SP2 Polyclonal Antibody (Product # PA5-35984) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SP2 Polyclonal Antibody (Product # PA5-35984). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP2 Polyclonal Antibody (Product # PA5-35984) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SP2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SP2 Polyclonal Antibody (Product # PA5-35984) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SP2 was performed in paraffin-embedded human endometrial carcinoma tissue using SP2 Polyclonal Antibody (Product # PA5-35984) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SP2 Polyclonal Antibody immunoprecipitates SP2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 µg whole cell lysate/extract of SP2 protein expressing Raji cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SP2 by 3 µg of SP2 Polyclonal Antibody (Product # PA5-35984) 5% SDS-PAGE The immunoprecipitated SP2 protein was detected by SP2 Polyclonal Antibody (Product # PA5-35984) diluted at 1 : 500. Anti-rabbit IgG (HRP) was used as a secondary reagent.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6 Competitive combination of DUSP5 and SCP1/2 with SMAD1. A-G, Co-IP assay was performed to determine the interaction between SCP1 and SMAD1, and the interaction between SCP2 and SMAD1 in NC, sh DUSP5 -1 and sh DUSP5 -3 HEK293T cells. H-N, The interaction between SCP1 and SMAD1, and the interaction between SCP2 and SMAD1 in vector and DUSP5 HEK293T cells was detected by Co-IP experiment. Data represented were repeated three times. Co-IP, coimmunoprecipitation; DUSP5, dual-specificity phosphatase 5; NC, negative control for sh DUSP5 -1 and sh DUSP5 -3; SCP1, small C-terminal phosphatase 1; SCP2, small C-terminal phosphatase 2; vector, negative control for DUSP5