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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
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- Product number
- BS-8507R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Hairless Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1:Siha cell lysates; Lane 2: A549 cell lysates probed with Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1:Siha cell lysates; Lane 2: A549 cell lysates probed with Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- An ELISA was performed against antigen bs-8307P at a dilution of 2 µg/100 µl. The following serial dilutions using the Primary antibody against antiserum were used: 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000. The secondary antibody was an HRP conjugated Goat Anti-Rabbit IgG (bs-0294R-HRP) at a dilution of 1:5,000. TMB staining and a microplate reader at 450 nm was used for detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
