Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-72585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ATF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- Lot Dependent
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATF7 in HeLa cells. Samples were fixed with 4% paraformaldehyde (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATF7 polyclonal antibody (Product # PA5-72585) using a dilution of 1:25 for 1 h at 37?). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ATF7 in HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with ATF7 polyclonal antibody (Product # PA5-72585) followed by a FITC conjugated goat anti-rabbit secondary antibody.