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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 702901 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD179b Recombinant Rabbit Monoclonal Antibody (8H16L35)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 8H16L35
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of JM1 (Lane 1), Daudi (Lane 2), EB2 (Lane 3) and IM-9 (Lane 4). The blot was probed with Anti-CD179b Recombinant Rabbit Monoclonal Antibody (Product # 702901, 1:10000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 23 kDa corresponding to CD179b was observed in relevant cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, JM1 cells were fixed and permeabilized for detection of endogenous CD179b using Anti-CD179b Recombinant Rabbit Monoclonal Antibody (Product # 702901,1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates membrane localization of CD179b in JM1 cells. Panel e-h) shows no specific staining in Raji cells which is CD179b negative, demonstrating specificity. The images were captured at 60X magnification.
