MA5-34636

antibody from Invitrogen Antibodies

Targeting: CRMP1 DPYSL1, DRP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34636

Provider

Invitrogen Antibodies

Product name

CRMP1 Recombinant Rabbit Monoclonal Antibody (JU73-30)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: Mouse brain tissue, N2A, SHG-44, SH-SY5Y, rat brain tissue, mouse cerebellum tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU73-30

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRMP1 in mouse brain tissue lysate. Samples were incubated with CRMP1 monoclonal antibody (Product # MA5-34636), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CRMP1 in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CRMP1 monoclonal antibody (Product # MA5-34636), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CRMP1 in SHG-44 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CRMP1 monoclonal antibody (Product # MA5-34636), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CRMP1 in N2A cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with CRMP1 monoclonal antibody (Product # MA5-34636), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CRMP1 in paraffin-embedded rat brain tissue. Samples were incubated with CRMP1 monoclonal antibody (Product # MA5-34636), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CRMP1 in paraffin-embedded mouse cerebellum tissue. Samples were incubated with CRMP1 monoclonal antibody (Product # MA5-34636), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CRMP1 in paraffin-embedded mouse brain tissue. Samples were incubated with CRMP1 monoclonal antibody (Product # MA5-34636), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CRMP1 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with CRMP1 monoclonal antibody (Product # MA5-34636) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.