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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA500493 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500493, RRID:AB_2182726
- Product name
- Anti-SCYL3 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
- Antibody type
- Monoclonal
- Description
- Anti-SCYL3 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SCYL3
- Isotype
- IgG
- Antibody clone number
- OTI4F10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCYL3 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SCYL3 mouse monoclonal antibody (TA500493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCYL3(RC204859).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500493, Dilution 1:50)
- Validation comment
- IHC
