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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA500493 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCYL3 Monoclonal Antibody (OTI4F10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI4F10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3. Positive lysates LY412474 (100 µg) and LC412474 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-SCYL3 monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-SCYL3 mouse monoclonal antibody (TA500493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCYL3(RC204859).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500493, Dilution 1:50)
