MAB2105

antibody from Novus Biologicals

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2105

Provider

Novus Biologicals

Product name

Rat Monoclonal Vimentin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots.

Reactivity

Human, Mouse, Rat

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice.

Wichaiyo S, Lax S, Montague SJ, Li Z, Grygielska B, Pike JA, Haining EJ, Brill A, Watson SP, Rayes J
Haematologica 2019 Aug;104(8):1648-1660

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RNA sequencing reveals upregulation of a transcriptomic program associated with stemness in metastatic prostate cancer cells selected for taxane resistance.

Cajigas-Du Ross CK, Martinez SR, Woods-Burnham L, Durán AM, Roy S, Basu A, Ramirez JA, Ortiz-Hernández GL, Ríos-Colón L, Chirshev E, Sanchez-Hernandez ES, Soto U, Greco C, Boucheix C, Chen X, Unternaehrer J, Wang C, Casiano CA
Oncotarget 2018 Jul 13;9(54):30363-30384

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Acute Drug Effects on the Human Placental Tissue: The Development of a Placental Murine Xenograft Model.

Verheecke M, Hermans E, Tuyaerts S, Souche E, Van Bree R, Verbist G, Everaert T, Cortès-Calabuig A, Van Houdt J, Van Calsteren K, Amant F
Reproductive sciences (Thousand Oaks, Calif.) 2018 Dec;25(12):1637-1648

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Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.

Procopio MG, Laszlo C, Al Labban D, Kim DE, Bordignon P, Jo SH, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski WW, Swartz MA, Brisken C, Lefort K, Dotto GP
Nature cell biology 2015 Sep;17(9):1193-204

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GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray.

Riess JW, West R, Dean M, Klimowicz AC, Neal JW, Hoang C, Wakelee HA
Anticancer research 2015 Feb;35(2):669-76

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Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures.

Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V
Investigative ophthalmology & visual science 2008 Dec;49(12):5325-31

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Vimentin by Western Blot. Detection of Human Vimentin by Western Blot.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Vimentin by Simple WesternTM. Detection of Human Vimentin by Simple WesternTM.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse and Rat Vimentin by Western Blot. Detection of Mouse and Rat Vimentin by Western Blot.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Vimentin in Human Tonsil. Vimentin in Human Tonsil.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. Detection of Vimentin in A172 Human Cell Line by Flow Cytometry.