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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F48162 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Vimentin Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 430-457 from the human protein was used as the immunogen for this anti-Vimentin antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the anti-Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. Vimentin is localized to the cytoskeleton.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
