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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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- Product number
- SM3166P - Provider product page
- Provider
- OriGene
- Product name
- Vimentin (VIM) mouse monoclonal antibody, clone VI-10, Purified
- Antibody type
- Monoclonal
- Description
- Vimentin (VIM) mouse monoclonal antibody, clone VI-10, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- VIM
- Isotype
- IgM
- Antibody clone number
- VI-10
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
Submitted references Monoclonal antibody VI-10 specific for vimentin.
Dráberová E, Zíková M, Dráber P
Folia biologica 1999;45(1):35-6
Folia biologica 1999;45(1):35-6
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2. Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 1. Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; BM753S), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 3. Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)
- Validation comment
- IP
