200-901-E54

antibody from Rockland Immunochemicals, Inc.

Targeting: VIM

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

200-901-E54

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-901-E54, RRID:AB_11183120

Product name

Anti-Vimentin (Chicken) Antibody - 200-901-E54

Antibody type

Polyclonal

Vial size

100 µl

Immunocytochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunofluorescence Microscopy of Chicken anti-Vimentin antibody. Tissue: Mixed neuron/glial cultures. Fixation: 0.5% PFA Antigen retrieval: not required. Primary antibody: Vimentin antibody at 1:500 for 1 h at RT. Secondary antibody: Fluorescein chicken secondary antibody at 1:10,000 for 45 min at RT. Localization: Vimentin is found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Staining: Vimentin Antibody as green fluorescent signal, GFAP Antibody as red, Vimentin and GFAP as yellow.

Sample type

IF Microscopy