Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- SM3128P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM3128P, RRID:AB_1008517
- Product name
- anti Vimentin
- Antibody type
- Monoclonal
- Antigen
- Pellet of pig brain cold stable proteins after depolymerization of microtubules
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- VI-01
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
Submitted references Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture.
Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions.
A common antigenic determinant of vimentin and desmin defined by monoclonal antibody.
Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V
Journal of biomedical materials research 2000 Mar 5;49(3):369-79
Journal of biomedical materials research 2000 Mar 5;49(3):369-79
Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions.
Bacáková L, Mares V, Lisá V, Svorcík V
Biomaterials 2000 Jun;21(11):1173-9
Biomaterials 2000 Jun;21(11):1173-9
A common antigenic determinant of vimentin and desmin defined by monoclonal antibody.
Dráberová E, Dráber P, Havlícek F, Viklický V
Folia biologica 1986;32(5):295-303
Folia biologica 1986;32(5):295-303
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3. Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (VI-01)Lane 3,4: immunostaining with anti-human Vimentin (VI-RE/1; SM3129P)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547. Nuclei are stained with DAPI (blue).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-01) Dyomics 547. Nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 4. Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa.Lr = lysate (reducing conditions)Lnr = lysate (non-reducing conditions)IPr = immunoprecipitate (reducing conditions)IPnr = immunoprecipitate (non-reducing conditions)
