25155-1-AP

antibody from Invitrogen Antibodies

Targeting: ATG2B C14orf103, FLJ10242

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25155-1-AP

Provider

Invitrogen Antibodies

Product name

ATG2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RDYVRVMDM GLLELTITAV KSDSDGEQTE PRFELHCSSD VVHIRTCSDS CAALMNLIQY IASYGDLQTP NKADMKPGAF QRRSKVDSSG RSSSRGPVLP EADQQMLRDL MSDAMEEIDM QQGTSSVKPQ ANGVLDEKSQ IQEPCCSDLF LFPDESGNVS QESGPTYASF SHHFISDAMT GVPTENDDFC ILFAPKAAMQ EKEEEPVIKI MVDDAIVIRD NYFSLPVNKT DTSKAPLHFP IPVIRYVVKE VS (1304-1555 aa encoded by B C015016)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25155-1-AP ( ATG2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25155-1-AP (ATG2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 25155-1-AP (ATG2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 25155-1-AP ( ATG2B antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 25155-1-AP ( ATG2B antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).