12230-2-AP

antibody from Invitrogen Antibodies

Targeting: RBBP9 Bog

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12230-2-AP

Provider

Invitrogen Antibodies

Product name

RBBP9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASPSKAVI VPGNGGGDVT THGWYGWVKK ELEKIPGFQC LAKNMPDPIT ARESIWLPFM ETELHCDEKT IIIGHSSGAI AAMRYAETHR VYAIVLVSAY TSDLGDENER ASGYFTRPWQ WEKIKANCPY IVQFGSTDDP FLPWKEQQEV ADRLETKLHK FTDCGHFQNT EFHELITVVK SLLKVPA (1-186 aa encoded by BC015938)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human spleen tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12230-2-AP (RBBP9 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 BxPC-3 cells were stained with 0.2ug RBBP9 antibody (12230-2-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RBBP9 (IP:12230-2-AP, 3ug; Detection:12230-2-AP 1:300) with mouse lung tissue lysate 5160ug.