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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 11118-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEST Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MVRRDRLRR MREWWVQVGL LAVPLLAAYL HIPPPQLSPA LHSWKSSGKF FTYKGLRIFY QDSVGVVGSP EIVVLLHGFP TSSYDWYKIW EGLTLRFHRV IALDFLGFGF SDKPRPHHYS IFEQASIVEA LLRHLGLQNR RINLLSHDYG DIVAQELLYR YKQNRSGRLT IKSLCLSNGG IFPETHRPLL LQKLLKDGGV LSPILTRLMN FFVFSRGLTP VFGPYTRPSE SELWD (1-234 aa encoded by BC011908)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MEST (IP:11118-1-AP, 3ug; Detection:11118-1-AP 1:500) with PC-3 cells lysate 1720ug.
