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- Antibody Data
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- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NBP1-31029 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31029, RRID:AB_2188618
- Product name
- Rabbit Polyclonal Sirtuin 3/SIRT3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway.
Park JH, Burgess JD, Faroqi AH, DeMeo NN, Fiesel FC, Springer W, Delenclos M, McLean PJ
Molecular neurodegeneration 2020 Jan 13;15(1):5
Molecular neurodegeneration 2020 Jan 13;15(1):5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Sample(30 ug of whole cell lysate)A:Hep G2 10% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody at a dilution of 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Paraffin-embedded human endometrial carcinoma diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
