HPA001677

antibody from Atlas Antibodies

Targeting: GOLIM4 GIMPC, GOLPH4, GPP130, P138

Antibody data

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Validation data

Product number: HPA001677 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA001677, RRID:AB_1079017
Product name: Anti-GOLIM4
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human GOLIM4, Gene description: golgi integral membrane protein 4, Alternative Gene Names: GIMPC, GOLPH4, GPP130, P138, Validated applications: ICC, IHC, Uniprot ID: O00461, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.05 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

GOLIM4 protein structure - HPA001677 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human small intestine and skin tissues using Anti-GOLIM4 antibody. Corresponding GOLIM4 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human small intestine and skin tissues using HPA001677 antibody. Corresponding GOLIM4 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, liver, skin and small intestine using Anti-GOLIM4 antibody HPA001677 (A) shows similar protein distribution across tissues to independent antibody HPA002315 (B).
Antibody #2 product nr: HPA002315
Antibody provider: Atlas Antibodies

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, liver, skin and small intestine using Anti-GOLIM4 antibody HPA001677 (A) shows similar protein distribution across tissues to independent antibody HPA002315 (B).
Sample type: Human
Secondary Ab: Secondary Ab
Protocol: Protocol
Antibody #2 product nr: HPA002315
Antibody provider: Atlas Antibodies
Antibody Prod page URL: Antibody Prod page URL
Appendix: ?language=en&utm_campaign=antibodypedia

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human small intestine shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-GOLIM4 antibody HPA001677.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex using Anti-GOLIM4 antibody HPA001677.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human small intestine shows strong positivity in Golgi apparatus in glandular cells.
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons and glial cells.
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows moderate granular cytoplasmic positivity in a small subset of squamous epithelial cells.
Sample type: Human
Protocol: Protocol